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KMID : 0370220080520060441
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Yakhak Hoeji
2008 Volume.52 No. 6 p.441 ~ p.445
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Effect of Caffeic Acid on the Production of Reactive Oxygen Species in Raw 264.7 Cells
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Choi Byung-Chul
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Abstract
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To investigate effect of caffeic acid on the intracellular reactive oxygen species production, we used DHE for intracellular superoxide anion production, DCF for intracellular H2O2 production and DHR for intracellular hydroperoxide production in Raw 264.7 cells. DPPH assay showed that antioxidant activity of caffeic acid with 39.5 μM of IC50 values was similar to that of ascorbic acid with 41.3 μM of IC50 values. Caffeic acid dose-dependently inhibited silica-induced H2O2 and hydroperoxide production but did not affect superoxide anion production in Raw 264.7 cells, which suggest that antioxidant effect of caffeic acid acts on the post-step of superoxide anion. On the other hand, caffeic acid showed a potent antioxidant effect in lCuSO4-induced lipid peroxidation. Furthermore, plasma superoxide dismutase activity (3.43±0.23 U/ml) in 10 mg/kg caffeic acid-fed mice was significantly higher than that (2.32±0.24 U/ml) of control. From the above results, it is referred that caffeic acid appears to have potent anti-oxidant activity in both cell system and in vivo system.
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KEYWORD
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caffeic acid, reactive oxygen species, nitric oxide, superoxide dismutase
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